GSM2035727: FL-HSPC sh-HOXA7_infected experiment_2 (HOXA7 in shHOXA7g... - SRA

SRX1526342: GSM2035727: FL-HSPC sh-HOXA7_infected experiment_2 (HOXA7 in shHOXA7gene_exp.diff); Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 20.2M spots, 1G bases, 829.7Mb downloads

Submitted by: NCBI (GEO)

Study: RNA-seq expression data from FL-HSPCs after HOXA7 knockdown

PRJNA308425 • SRP068278 • All experiments • All runs

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HOXA7 regulates FL-HSPC self-renewal in vitro and in vivo. We profiled FL-HSPCs after HOXA7 knockdown, to assess the gene expression programs regulated by HOXA7. Overall design: CD34+CD38-CD43+CD90+ HSPCs were infected with lentiviral shRNA vector targeting HOXA7 (sh-HOXA7). The emtpy lentiviral vector (LKO) was used as a control. Cells were expanded on op9 for 5 days and selected for puromicin resistance for 3 days, than sorted for HSPC immunophenotype.

Sample: FL-HSPC sh-HOXA7_infected experiment_2 (HOXA7 in shHOXA7gene_exp.diff)

SAMN04395234 • SRS1244225 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: RNA was purified from sorted cells using RNeasy Mini Kit (QIAGEN), according to manufacturer's instructions. Ovation Rna-seq system System v2 (Nugen), followed by KAPA LTP Library Preparation Kit

Experiment attributes:

GEO Accession: GSM2035727

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 20.2M spots, 1G bases, 829.7Mb

Run	# of Spots	# of Bases	Size	Published
SRR3095994	20,209,200	1G	829.7Mb	2016-05-17

ID:: 2157613

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